Programma di Modern Techniques Of Protein Identification And Molecular Recognition Methods:
Molecular Recognition Methods Course Program:
Fundamentals of light- matter interaction. Electromagnetic spectrum. Structure of matter.
Absorption and luminescence phenomena. Absorption and fluorescence spectroscopy of
biological samples. Polarization of light. Linear and circular dichroism. Fluorescence
anisotropy. Infrared and Raman (SERS) spectroscopy.
Microscopy: Principles of optics and microscopy. Optical and confocal microscopy.
Ultraresolution methods, Probe scanning microscopy (STM, AFM, SNOM).
Classic experimental methods: Chromatography, Coimmunoprecipitation, Cross linking,
Two-hybrid assay, in yeast and bacteria. Fluorescent based methods, fragment
complementation.
Advanced experimental methods: Surface plasmon resonance (SPR), Atomic force
microscopy, Optical Tweezers, In vivo imaging (FRET, STED)
Overview of Computational Methods: Structural Modeling, Molecular Dynamics, Docking.
Final Exam : production of a written (report) or oral (presentation) on a scientific article
plus a short oral exam.
Specifications to be included in the written/oral report: Authors, laboratory, Biological
context, specific problem that they tackle. Methods used, Results, Interpretation. Overall
consistency and clarity, pitfalls, critical comments.
English
Italiano