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Programma di Modern Techniques Of Protein Identification And Molecular Recognition Methods:

Molecular Recognition Methods Course Program: Fundamentals of light- matter interaction. Electromagnetic spectrum. Structure of matter. Absorption and luminescence phenomena. Absorption and fluorescence spectroscopy of biological samples. Polarization of light. Linear and circular dichroism. Fluorescence anisotropy. Infrared and Raman (SERS) spectroscopy. Microscopy: Principles of optics and microscopy. Optical and confocal microscopy. Ultraresolution methods, Probe scanning microscopy (STM, AFM, SNOM). Classic experimental methods: Chromatography, Coimmunoprecipitation, Cross linking, Two-hybrid assay, in yeast and bacteria. Fluorescent based methods, fragment complementation. Advanced experimental methods: Surface plasmon resonance (SPR), Atomic force microscopy, Optical Tweezers, In vivo imaging (FRET, STED) Overview of Computational Methods: Structural Modeling, Molecular Dynamics, Docking.   Final Exam : production of a written (report) or oral (presentation) on a scientific article plus a short oral exam. Specifications to be included in the written/oral report: Authors, laboratory, Biological context, specific problem that they tackle. Methods used, Results, Interpretation. Overall consistency and clarity, pitfalls, critical comments.
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