Genetics And Genetic Technology 2021/2022

Program of the Course: Genetics and Genetic Technologies

Genotype-Phenotype

Mendel: monohybrid and dihybrid crosses

Mendelian eredity in man

Chromosomal theory of inheritance

Meiosis and mitosis

Sex linked inheritance (Morgan)

Sex determination

X-linked inheritance in Hybrids

Anomalous segregation

One gene-one enzyme Hypothesis (Beadle and Tatum)

Epistasis, suppression, complementation

Lethal genes

Linkage and recombination

Interference

DNA structure and function

Replication, transcription, translation

Genetics of Bacteria and Phages

Plasmids and transposons

Mutations: origin, selection

Chromosome Translocation

The Regulation of Gene expression in Prokaryotes and Eukaryotes

The lactose operon, the Trp operon, the Ara operon. 

DNA recombinant technology: restriction enzymes, vectors, DNA cloning, selection strategies, electrophoresis, replica plating, PCR, DNA sequencing (Sanger)